Resource Type

Journal Article 124

Conference Videos 1

Year

2023 15

2022 24

2021 9

2020 19

2019 4

2018 4

2017 11

2016 1

2015 2

2014 5

2013 3

2012 8

2011 5

2010 3

2009 1

2008 3

2007 3

2001 1

open ︾

Keywords

fluorescence 12

Fluorescence 4

DX pile 3

Free nitrous acid 2

mechanical properties 2

numerical analysis 2

-ECBM 1

1 1

1p32.3 deletion 1

1q21 gain 1

2-arylvinyl 1

2n gamete 1

3-propanediol 1

4Pi nanoscopy 1

4Pi-SMLM 1

5-dimethylpyridine 1

In situ database 1

In situ pyrolysis 1

in-situ characterization 1

open ︾

Search scope:

排序： Display mode:

Potential unreliability of ALK variant allele frequency in the efficacy prediction of targeted therapy in NSCLC

Frontiers of Medicine 2023, Volume 17, Issue 3,   Pages 493-502 doi: 10.1007/s11684-022-0946-x

Abstract: Anaplastic lymphoma kinase (ALK) is the most common fusion gene involved in non-small cell lung cancer (NSCLC), and remarkable response has been achieved with the use of ALK tyrosine kinase inhibitors (ALK-TKIs). However, the clinical efficacy is highly variable. Pre-existing intratumoral heterogeneity (ITH) has been proven to contribute to the poor treatment response and the resistance to targeted therapies. In this work, we investigated whether the variant allele frequencies (VAFs) of ALK fusions can help assess ITH and predict targeted therapy efficacy. Through the application of next-generation sequencing (NGS), 7.2% (326/4548) of patients were detected to be ALK positive. On the basis of the adjusted VAF (adjVAF, VAF normalization for tumor purity) of four different threshold values (adjVAF < 50%, 40%, 30%, or 20%), the association of ALK subclonality with crizotinib efficacy was assessed. Nonetheless, no statistical association was observed between median progression-free survival (PFS) and ALK subclonality assessed by adjVAF, and a poor correlation of adjVAF with PFS was found among the 85 patients who received first-line crizotinib. Results suggest that the ALK VAF determined by hybrid capture-based NGS is probably unreliable for ITH assessment and targeted therapy efficacy prediction in NSCLC.

Keywords： ALK fusion     next-generation sequencing     fluorescence in situ hybridization     immunohistochemistry    

HTML PDF Collect

Niche Differentiation of Phenol-Degrading Microorganisms in UASB Granular Sludge as Revealed by Fluorescencein situ Hybridization Article

Kengo Kubota, Kei Igarashi, Masayoshi Yamada, Yasuyuki Takemura, Yu-You Li, Hideki Harada

Engineering 2022, Volume 9, Issue 2,   Pages 61-66 doi: 10.1016/j.eng.2021.05.012

Abstract: harvested from an anaerobic sludge blanket reactor treating phenolic wastewater was investigated using fluorescencein situ hybridization (FISH) and clone library construction.

Keywords： Cryptanaerobacter     Fluorescence     in situ     hybridization     Anaerobic phenol degradation     Syntrophorhabdaceae    

HTML PDF Collect

Short-term effects of excessive anaerobic reaction time on anaerobic metabolism of denitrifying polyphosphate- accumulating organisms linked to phosphorus removal and N

Gang GUO, Yayi WANG, Chong WANG, Hong WANG, Mianli PAN, Shaowei CHEN

Frontiers of Environmental Science & Engineering 2013, Volume 7, Issue 4,   Pages 616-624 doi: 10.1007/s11783-013-0505-4

Abstract: The fluorescence in situ hybridization (FISH) analysis revealed that the sludge was mainly dominated

Keywords： Denitrifying phosphorus removal     anaerobic reaction time     nitrous oxide     polyhydroxyalkanoate     free nitrous acid     fluorescencein-situ hybridization    

HTML PDF Collect

hydrophobic environment triggering reactive fluorescence probe to real-time monitor mitochondrial DNA

Frontiers of Chemical Science and Engineering 2022, Volume 16, Issue 1,   Pages 92-102 doi: 10.1007/s11705-021-2063-9

Abstract: Herein, we constructed an in situ hydrophobic environment-triggering reactive fluorescence probeThus, MBI-CN can be hydrolyzed to generate MBI-CHO in an in situ hydrophobic environment withMeanwhile, MBI-CHO immediately emitted a significative fluorescence signal changes at 437 and 553 nm

Keywords： hydrolysis reaction     mitochondrial DNA damage     in situ hydrophobic environment trigger     fluorescence    

HTML PDF Collect

Short-term effect of temperature variation on the competition between PAOs and GAOs during acclimation period of an EBPR system

Nanqi REN, Han KANG, Xiuheng WANG, Nan LI

Frontiers of Environmental Science & Engineering 2011, Volume 5, Issue 2,   Pages 277-282 doi: 10.1007/s11783-010-0226-x

Abstract: PAOs and GAOs were analyzed by fluorescent in situ hybridization (FISH), and intracellular polyphosphate

Keywords： fluorescent in situ hybridization (FISH)     tetrad forming organisms (TFOs)     temperature variation     enhanced    

HTML PDF Collect

Comments on “Determination of heterozygosity for avirulence/virulence loci through sexual hybridization

Ralf T. Voegele

Frontiers of Agricultural Science and Engineering 2017, Volume 4, Issue 1,   Pages 121-122 doi: 10.15302/J-FASE-2017142

HTML PDF Collect

Determination of heterozygosity for avirulence/virulence loci through sexual hybridization of

Yuan TIAN,Gangming ZHAN,Xia LU,Jie ZHAO,Lili HUANG,Zhensheng KANG

Frontiers of Agricultural Science and Engineering 2017, Volume 4, Issue 1,   Pages 48-58 doi: 10.15302/J-FASE-2016114

Abstract: Wheat stripe rust caused by f. sp. is one of the most devastating diseases of wheat worldwide and resistant cultivars are vital for its management. Therefore, investigating the heterozygosity of the pathogen is important because of rapid virulence changes in isolates heterozygous for avirulence/virulence. An isolate of f. sp. was selfed on to determine the heterozygosity for avirulence/virulence loci. One hundred and twenty progeny isolates obtained from this selfing were phenotyped using 25 lines of wheat containing genes and genotyped with 96 simple sequencing repeat markers, with 51 pathotypes and 55 multi-locus genotypes being identified. All of these were avirulent on lines with , , , and and virulent on lines with , and , indicating that the parental isolate was homozygously avirulent or homozygously virulent for these loci. Segregation was found for wheat lines with , , , , , , , , , , , , , , , and . The 17 cultivars to which the was identified as heterozygous with respect to virulence/avirulence should not be given priority in breeding programs to obtain new resistant cultivars.

Keywords： Puccinia striiformis f. sp. tritici     selfing     heterozygosity     virulence inheritance    

HTML PDF Collect

Effect of fiber hybridization on energy absorption and synergy in concrete

Ahmadreza RAMEZANI, Mohammad Reza ESFAHANI

Frontiers of Structural and Civil Engineering 2019, Volume 13, Issue 6,   Pages 1338-1349 doi: 10.1007/s17009-019-0558-2

Abstract: main concentration being on energy absorption characteristics of concrete and the efficacy of fiber hybridization

Keywords： hybrid fiber-reinforced concrete     synergy     toughness     steel fibers     polypropylene fibers    

HTML PDF Collect

Development and application of ex-situ presulfurization technology for hydrotreating catalysts in China

Yulan GAO, Xiangchen FANG, Zhenmin CHENG

Frontiers of Chemical Science and Engineering 2011, Volume 5, Issue 3,   Pages 287-296 doi: 10.1007/s11705-010-0529-2

Abstract: The development and application of ex-situ presulfurization (EPRES) technology for hydrotreating catalysts

Keywords： ex-situ presulfurization     in situ presulfurization     hydrotreating catalyst     sulfur utilization ratio    

HTML PDF Collect

Synthesis and fluorescence behavior of 2,5-diphenyl-1,3,4-oxadiazole-containing bismaleimides and bissuccinimides

Xin ZHANG, Zichen LI

Frontiers of Chemical Science and Engineering 2013, Volume 7, Issue 4,   Pages 381-387 doi: 10.1007/s11705-013-1359-9

Abstract: Almost no or very weak fluorescence was observed for these bismaleimides, however, the bissuccinimidesshow a strong fluorescence.by UV-vis absorption and fluorescence emission spectroscopy.-Bissuccinimide displays increasing fluorescence quantum yields with red shifts of 22–24 nm, comparedPolymerizable C=C bonds play a key role in the intramolecular fluorescence quenching.

Keywords： fluorescence     bismaleimide     oxadiazole     succinimide    

HTML PDF Collect

Polyolefin-nanocomposites with special properties by in-situ polymerization

Walter Kaminsky

Frontiers of Chemical Science and Engineering 2018, Volume 12, Issue 3,   Pages 555-563 doi: 10.1007/s11705-018-1715-x

Abstract:

Exceptionally strong polyolefin nanocomposites are synthesized by in-situ polymerization using

Keywords： polyolefin nanocomposites     metallocenes     methylaluminoxane    

HTML PDF Collect

Synthesis and characterization of 2,5-bis[4-(2-arylvinyl)phenyl]-1,3,4-oxadiazoles with two-photon fluorescence

ZHU Xiaoqin, QIAN Ying, LU Zhifeng

Frontiers of Chemical Science and Engineering 2007, Volume 1, Issue 4,   Pages 381-384 doi: 10.1007/s11705-007-0069-6

Abstract: phenyl]-1,3,4-oxadiazoles that exhibit strong two-photon absorption and enhanced two-photon excited fluorescence-conjugated bridging unit can enhance the two-photon absorption and two-photon fluorescence.

Keywords： 2-arylvinyl     trans-vinylene character     effective     fluorescence     push-core-pull-core-push    

HTML PDF Collect

Carbon dots-based fluorescence sensor for two-photon imaging of pH in diabetic mice

Frontiers of Chemical Science and Engineering 2023, Volume 17, Issue 3,   Pages 298-306 doi: 10.1007/s11705-022-2212-9

Abstract: The carbon dots-based sensor (CA-CDs) exhibited pH-dependent increase in fluorescence intensity and showedNotably, the fluorescence sensor has a reversible response to pH change.

Keywords： carbon dots     two-photon imaging     pH     diabetic mice    

HTML PDF Collect

Insight into fluorescence properties of 14 selected toxic single-ring aromatic compounds in water: Experimental

Muhammad Farooq Saleem Khan, Jing Wu, Cheng Cheng, Mona Akbar, Chuanyang Liu, Bo Liu, Jian Shen, Yu Xin

Frontiers of Environmental Science & Engineering 2020, Volume 14, Issue 3, doi: 10.1007/s11783-020-1219-z

Abstract: The fluorescence peak location of 14 compounds interpreted at protein-like region.The fluorescence excitation-emission matrix (EEMs) spectrophotometry is a useful method to identify organicThis study provides a detailed insight into the fluorescence properties of the 14 selected toxic single-ringThe fluorescence of halogenated benzenes illustrates an increase in the HOMO-LUMO energy gap and a decreaseThe understanding of fluorescence and photophysical properties are of great importance in the identification

Keywords： Fluorescence     Photophysical properties     Effect of the substituent     Toxic aromatic compounds    

HTML PDF Collect

Cd-tolerant electrochemically active bacteria in MECs toward exotic Cr(VI) based on the sensing of fluorescence

Xia Hou, Liping Huang, Peng Zhou, Hua Xue, Ning Li

Frontiers of Environmental Science & Engineering 2018, Volume 12, Issue 4, doi: 10.1007/s11783-018-1057-4

Abstract:

Cell membrane of indigenous Cd-tolerant EAB harbored more cadmium than chromium.

Indigenous Cd-tolerant EAB cytoplasm located more chromium than cadmium.

Simultaneously quantitatively imaging Cd(II) and Cr(III) ions in EAB was achieved.

Current accelerated the harboring of cadmium in EAB at an initial 2 h.

Current directed the accumulation of more chromium in EAB over time.

Keywords： Microbial electrolysis cell     Electrochemically active bacteria     Cd-tolerant bacteria     Cd(II) and Cr(VI)     Fluorescence    

HTML PDF Collect

Title Author Date Type Operation

Potential unreliability of ALK variant allele frequency in the efficacy prediction of targeted therapy in NSCLC

Journal Article

Niche Differentiation of Phenol-Degrading Microorganisms in UASB Granular Sludge as Revealed by Fluorescencein situ Hybridization

Kengo Kubota, Kei Igarashi, Masayoshi Yamada, Yasuyuki Takemura, Yu-You Li, Hideki Harada

Journal Article

Short-term effects of excessive anaerobic reaction time on anaerobic metabolism of denitrifying polyphosphate- accumulating organisms linked to phosphorus removal and N

Gang GUO, Yayi WANG, Chong WANG, Hong WANG, Mianli PAN, Shaowei CHEN

Journal Article

hydrophobic environment triggering reactive fluorescence probe to real-time monitor mitochondrial DNA

Journal Article

Short-term effect of temperature variation on the competition between PAOs and GAOs during acclimation period of an EBPR system

Nanqi REN, Han KANG, Xiuheng WANG, Nan LI

Journal Article

Comments on “Determination of heterozygosity for avirulence/virulence loci through sexual hybridization

Ralf T. Voegele

Journal Article

Determination of heterozygosity for avirulence/virulence loci through sexual hybridization of

Yuan TIAN,Gangming ZHAN,Xia LU,Jie ZHAO,Lili HUANG,Zhensheng KANG

Journal Article

Effect of fiber hybridization on energy absorption and synergy in concrete

Ahmadreza RAMEZANI, Mohammad Reza ESFAHANI

Journal Article

Development and application of ex-situ presulfurization technology for hydrotreating catalysts in China

Yulan GAO, Xiangchen FANG, Zhenmin CHENG

Journal Article

Synthesis and fluorescence behavior of 2,5-diphenyl-1,3,4-oxadiazole-containing bismaleimides and bissuccinimides

Xin ZHANG, Zichen LI

Journal Article

Polyolefin-nanocomposites with special properties by in-situ polymerization

Walter Kaminsky

Journal Article

Synthesis and characterization of 2,5-bis[4-(2-arylvinyl)phenyl]-1,3,4-oxadiazoles with two-photon fluorescence

ZHU Xiaoqin, QIAN Ying, LU Zhifeng

Journal Article

Carbon dots-based fluorescence sensor for two-photon imaging of pH in diabetic mice

Journal Article

Insight into fluorescence properties of 14 selected toxic single-ring aromatic compounds in water: Experimental

Muhammad Farooq Saleem Khan, Jing Wu, Cheng Cheng, Mona Akbar, Chuanyang Liu, Bo Liu, Jian Shen, Yu Xin

Journal Article

Cd-tolerant electrochemically active bacteria in MECs toward exotic Cr(VI) based on the sensing of fluorescence

Xia Hou, Liping Huang, Peng Zhou, Hua Xue, Ning Li

Journal Article